Effects of dietary guava leaf aqueous extract supplementation on growth, antioxidant capacity, and non-specific immunity in mud crab <i>Scylla paramamosain</i>

Mud crab ( Scylla paramamosain ) fed five different diets with varying concentrations of guava leaf aqueous extract (0 mg·kg –1 , 80 160 320 and 640 for 30 days. crabs in the guava-leaf groups outperformed control group terms survival rates (SR), weight gain (WGR), specific growth (SGR). When compared to group, mud had significantly higher levels lipase (LPS), pepsin, lysozyme (LZM), superoxide dismutase (SOD), acid phosphatase (ACP), glutathione (GSH) P &lt; 0.05 ). The amylase (AMS) activity was decreased all experimental Malondialdehyde (MDA) content hepatopancreas were reduced Additionally, real-time PCR results illustrated that expression GPx3 CAT JNK considerably increased In groups, SOD genes greater than ), which consistent level activity. GST P53 gene up-regulated Overall, addition -320 feed promoted growth, enhanced activities digestive antioxidant enzymes, strengthened immunity.